LINE-1 protein localization and functional dynamics during the cell cycle

نویسندگان

  • Paolo Mita
  • Aleksandra Wudzinska
  • Xiaoji Sun
  • Joshua Andrade
  • Shruti Nayak
  • David J Kahler
  • Sana Badri
  • John LaCava
  • Beatrix Ueberheide
  • Chi Y Yun
  • David Fenyö
  • Jef D Boeke
چکیده

LINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology. However, a clear understanding of L1's lifecycle and the processes involved in restricting its insertion and intragenomic spread remains elusive. Here we identify modes of L1 proteins' entrance into the nucleus, a necessary step for L1 proliferation. Using functional, biochemical, and imaging approaches, we also show a clear cell cycle bias for L1 retrotransposition that peaks during the S phase. Our observations provide a basis for novel interpretations about the nature of nuclear and cytoplasmic L1 ribonucleoproteins (RNPs) and the potential role of DNA replication in L1 retrotransposition.

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عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2018